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KMID : 0363320180390061181
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Journal of Korean Oriental Internal Medicine
2018 Volume.39 No. 6 p.1181 ~ p.1190
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The Effect of Alisma canaliculatum and Polyporus umbellatus Extracts on Adipogenic Differentiation of Human Bone Marrow Derived Mesenchymal Stromal Stem cells
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Yu Sung-Ryul
Kim Si-Hyun
Shin Seon-Mi
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Abstract
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Objective: This study investigated the effect of purified medical herb extracts such as Alisma canaliculatum and Polyporus umbellatus on adipogenic differentiation of human bone marrow derived mesenchymal stromal stem cells (hBMSCs).
Methods: Two different medical herb were extracted using hot distilled water. The optimal concentration of extracts were fixed at 100 ng/ml by means of cell viability and cytotoxic assay. To test the adipogenic differentiation ability of extracts, we induced the adipogenesis of hBMSCs for 21 days. At day 5, the cell was harvested to check mRNA and protein expression level of adipogenic related factors. The efficacy of lipid droplet formation was evaluated using the oil-red O staining method at days 21.
Results: Two different medical herb extracts have no toxicity on hBMSCs. And two different medical herb extracts significantly decreased formation of lipid droplet compared with control groups in hBMSCs. The A. canaliculatum extract group showed the lowest mRNA and protein expression level of adipossgenic related transcription factors. This data suggests that extract of A. canaliculatum and P. umbellata decrease the adipogenic differentiation of hBMSCs.
Conclusions: Our findings indicate that water-extract of A. canaliculatum and P. umbellata will be useful therapeutic reagents for prevention of obesity related disease such as diabetes, hyperlipidemia, coronary artery disease, and osteoporosis.
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KEYWORD
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hBMSCs, Adipogenic differentiation, Alisma canaliculatum, Polyporus umbellata
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